Mapa de ligamiento del gen Endotelina-1 en el cromosoma 2 del pollo

Juana Moncaleano-Vega

Resumen


El objetivo de este estudio fue asignar por mapa ligamiento el gen Endotelina-1 (END1) al cromosoma 2 del pollo (GGA2). Los microsatélites MCW0314, MCW0245, ADL0164, MCW0264 y el diseño de cebadores fueron usados para la amplificación de secuencias específicas de END1. La técnica PCR-SSCP y el uso de geles denaturantes permitió la tipificación de los genotipos. Para la construcción del mapa se usaron los genotipos identificados en una población de 200 pollos Cobb500 utilizando el programa Mapmaker® version 3.0b. El resultado es un mapa del GGA2 donde END1 está ligado a los microsatélites reportados en un locus de un carácter cuantitativo (QTL) para susceptibilidad de los pollos de engorde a desarrollar el síndrome de hipertensión pulmonar.


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