Resumen
El objetivo de este estudio fue asignar por mapa ligamiento el gen Endotelina-1 (END1) al cromosoma 2 del pollo (GGA2). Los microsatélites MCW0314, MCW0245, ADL0164, MCW0264 y el diseño de cebadores fueron usados para la amplificación de secuencias específicas de END1. La técnica PCR-SSCP y el uso de geles denaturantes permitió la tipificación de los genotipos. Para la construcción del mapa se usaron los genotipos identificados en una población de 200 pollos Cobb500 utilizando el programa Mapmaker® version 3.0b. El resultado es un mapa del GGA2 donde END1 está ligado a los microsatélites reportados en un locus de un carácter cuantitativo (QTL) para susceptibilidad de los pollos de engorde a desarrollar el síndrome de hipertensión pulmonar.
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- Ariza, F., Harrison B., and Drinkwater D., 2001. The assignment by
- linkage mapping of four genes from human chromosome 22
- to bovine chromosome 5 and 17. Animal Genetics, 32, 371-374.
- Binder, A., 2007. A review of the genetic of essential hypertension.
- Current Opinion in Cardiology 22, 176-184.
- Buzała, M., Janicki, B., Czarnecki R. 2015. Consequences of
- different growth rates in broiler breeder and layer hens
- on embryogenesis, metabolism and metabolic rate: A
- review. Poultry Science; 94 (4): 728-733. DOI: 10.3382/ps/
- pev015
- Flores, V. N. 2013. Endothelin-1: intrinsic vasoconstrictor vascular
- endothelial. Revista Med 21(2): 64-78. http://www.scielo.org.
- co/pdf/med/v21n2/v21n2a08.pdf
- Gomez, A.P., Moreno, M.J., Iglesias, A., Coral, P.X., and
- Hernández, A., 2007. Endothelin 1, its endothelin type A
- receptor, connective tissue growth factor, and adrenomedullin
- expression in longs of pulmonary hypertension and
- nonhypertensive chickens. Poultry Science 86, 909-916.
- Groenen, M.A.M., Cheng, H.H., Bumstesd, N., Benkel, B.F.,
- Elwood, B.W., Burke, B., Burt, D.W., Crittenden, L.B.,
- Dodgson, J., Hillel, J., Lamont, S., Ponce de Leon, A., Soller,
- M., Takahashi, H., and Vignal, A., 2000. A consensus linkage
- map of the chicken genome. Genome Research 10,137-147.
- http://biotools.umassmed.edu/bioapps/primer3_www.cgi.
- Disponible en: http://www.ensembl.org/Gallus_gallus/
- Gene/Summary?db=otherfeatures;g=420854;r=2:62883381-62888254;t=XM_418943.4 (Consultado 09-07-2016)
- Hassanzadeh, M., Buyse, J., Toloei, T. and Eddy Decuypere. 2014.
- Ascites Syndrome in Broiler Chickens: A Review on the Aspect
- of Endogenous and Exogenous Factors Interactions. Japan
- Poultry Science Association 51: 229-241. http://www.jstage.
- jst.go.jp/ browse/ jpsa doi:10.2141/ jpsa.0130063
- Inesta, R., Guino, E., Moreno, V., 2005. Statistical analysis of genetic
- polymorphisms in epidemiological studies. Gaceta Sanitaria
- , 333-341.
- Jin, J.J., Nakura J., Wu, Z., Yamamoto, M., Abe, M., Tabara, Y.,
- Yamamoto Y., Igase, M., Kohara, K., Miki, T., 2003. Association
- of endothelin-1 gene variant with hypertension. Hypertension
- , 163-167.
- Kalmara, I. D., Vanrompay, D., Janssensa, G. P. J. 2013. Broiler
- ascites syndrome: Collateral damage from efficient feed to
- meat conversion. The Veterinary Journal 92 (2): 169-174.
- http://doi.org/10.1016/j.tvjl.N2013.03.011
- Lee, H., Kim, HK., Won, H. et al. 2016. Genetic relationship
- between an endothelin 1 gene polymorphism and lead-related
- high blood pressure. Molecular and Cellular Toxicology 12 (2):
- Doi:10.1007/s13273-016-0014-1
- Moncaleano-Vega, J., Ariza, F., and Hernández A. 2013.
- Association of NOS3 and HIF1αGene Polymorphisms with
- the Susceptibility of Broiler Chickens to Develop Hypoxic
- Pulmonary Hypertension. American Journal of Hypertension
- Research 1.1, 17-22. DOI: 10.12691/ajhr-1-1-4
- Rabie, T.S.K.M., Crooijmans, R.P.M.A., Bovenhuis, H., Vereijken,
- A.L.J., Veenendaal, T., Van der Poel, J.J., et al. 2005. Genetic
- mapping of quantitative trait loci affecting susceptibility
- in chicken to develop pulmonary hypertension syndrome.
- Animal Genetics 36, 468-476.
- Resnyk, C. W., Chen, C., Huang, H., Wu, CH., Simon, J., Le BihanDuval, E. et al. 2015. RNA-Seq Analysis of Abdominal Fat in
- Genetically Fat and Lean Chickens Highlights a Divergence in
- Expression of Genes Controlling Adiposity, Hemostasis, and
- Lipid Metabolism. PLoS ONE 10(10): e0139549. https://doi.
- org/10.1371/journal.pone.0139549
- Schmid, M., Nanda I., Guttenbach, M., Steinlein, C., Hoehn, H.,
- Schartl, M., Haaf, T., et al., 2000. First report on chicken genes
- and chromosomes 2000. Cytogenetic Cell genetic 90, 169-218.
- Tanaka, C., Kamide, K., Takiuchi, S., Kawano, Y., and Miyata,
- T., 2004. Evaluation of the Lys198Asn and 134delA genetic
- polymorphisms of the Endothelin-1 gene. Hypertens Research.
- (5):367-71.
- Wideman, R. F., Rhoads, D. D., Erf, G. F., Anthony N. B. 2013.
- Pulmonary arterial hypertension (ascites syndrome) in
- broilers: A review. Poultry Science 92 (1): 64-83. DOI: 10.3382/
- ps.2012-02745